Figure 4. Subcellular localization of slc24a5. Human MNT1 cells transfected with (A) GFP-tagged zebrafish slc24a5 (green) and (B) HA-tagged slc24a5 (red) clearly show intracellular expression. (C) HA-tagged D3 dopamine receptor localizes to the plasma membrane in MNT1 cells (red). 4′,6′-diamidino-2-phenylindole (DAPI) counterstain was used to visualize nuclei (blue). Scale bars in (A) and (B), 10 mm; in (C), 5 mm. (D) Rescue of dark pigmentation in a melanophore of a golden embryo by HA-tagged slc24a5. These dark cells appear in golden embryos injected with the HA-tagged construct, but not in mock-injected embryos. Scale bar, 10 mm. (E) Model for calcium accumulation in melanosomes.