Figure 2 (D-M). Rescue and morpholino knockdown establish slc24a5 as the golden gene. Lateral view of eye (D) and dorsal view of head (E) of 72-hpf wildtype embryos. (F and G) golb1 pigmentation pattern at 72 hpf, showing lightly pigmented cells. (H and I) 72 hpf golb1 larva injected with PAC215f11 show mosaic rescue; arrow identifies a heavily pigmented melanophore. (J and K) 72-hpf golb1 larva injected with full-length zebrafish slc24a5 RNA. (L and M) 72-hpf golb1 larvae injected with full-length human European (Thr 111 ) SLC24A5 RNA. Rescue with the ancestral human allele (Ala 111 ) is shown in fig. S4. Rescue in RNA-injected embryos is more apparent in melanophores (K) and (M) than in RPE.