Sea lamprey brain shows evolution of neurotransmitter co-localization in vertebrates

Figure2

Figure 2. Photomicrographs of transverse sections of the brain showing DA (red) and GABA (green) immunoreactivities. (A) Immunoreactivities of both DA and GABA in the caudal rhombencephalon appear yellow (A’) Immunoreactivity of DA alone in the caudal rhombencephalon (A’’) Immunoreactivity of GABA alone in the caudal rhombencephalon (B-D) Closeup of the arrowhead in A-A’’ which are focused on double labeled thin fibres (E) Immunoreactivities of both DA and GABA in the ventral isthmus appear yellow (E’) Immunoreactivity of DA alone in the ventral isthmus (E’’) Immunoreactivity of GABA alone in the ventral isthmus (F) Immunoreactivities of both DA and GABA in the ventral hypothalamus appear yellow (G) Immunoreactivity of DA alone in the ventral hypothalamus, close-up (E’’) Immunoreactivity of GABA alone in the ventral hypothalamus, close-up

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