Fertilization: Avoiding Polyspermy

The first issue that an egg and a sperm of any organism type face in successfully producing an embryo is the possibility of polyspermy.  Polyspermy is the fertilization of an egg by multiple sperm, and the results of such unions are lethal.

If multiple sperm fertilize an egg, the embryo inherits multiple paternal centrioles.  This causes competition for extra chromosomes and results in the disruption of the creation of the cleavage furrow, thus causing the zygote to die.  As an important model organism in the study of fertilization and embryonic development, polyspermy in sea urchins has been studied in detail.  The sea urchin’s methods of polyspermy prevention have been broken down into two main pathways.  These two primary pathways are known as the fast block and the slow block to polyspermy.

Fast Block

After the sperm’s receptors come into contact with the egg’s jelly layer and the acrosomal enzymes are released and break down the jelly layer, the sperm head comes into contact with the vitelline and plasma membranes of the egg.  When the two plasma membranes contact one another, signals in the egg are initiated.

First, Na+ channels in the egg open, allowing Na+ to flood into the egg.  This causes a depolarization of the egg from it’s normal resting potential of -70 mV.

While depolarization is occurring, the remainder of the jelly layer is dissolving.  With the dissolution of the jelly layer and the depolarization of the plasma membrane, the first block to preventing fertilization by multiple sperm is put into place.

These two simple changes are part of the first block to polyspermy, known as the fast block.  Within 1/10th of a second of contact, the fast block to polyspermy is initiated.

Slow Block

While the first fast block is relatively simple and happens quickly, the second block to polyspermy, the slow block, takes place 10-60 seconds after the initial penetration of the egg’s plasma membrane by the sperm.  In this pathway, the physical layout of the membranes change.

Figure 1: This image, from LIFE: The Science of Biology, Purves et al, 1998, shows the step-by-step process of the slow block system.

Once the sperm has made its way through the jelly layer, receptors in the vitelline layer confirm that it is indeed a sea urchin sperm.  As fertilization happens entirely externally in sea urchin species, this step is to confirm that another organism’s sperm is not fertilizing the sea urchin egg.  Once the sperm is confirmed to be compatible, the plasma membranes of the sperm and egg fuse.

Fusion of the two membranes causes a release of inosital triphosphate.  The release of inosital triphosphate, or IP3, causes Ca2+ to be released into the egg’s cytoplasm.

Waiting in the cytoplasm are cortical vescicles, which are allowed to fuse with the egg’s plasma membrane and release their contents when the appropriate signal is sent.  Ca2+ released is that necessary signal, and the vesicles fuse with the membrane. The cortical vescicles contain enzymes which cause a number of changes to occur in the egg.

1.  The egg’s sperm receptors are removed

2.  H2O is absorbed, causing the membrane to swell and push the vitelline membrane away from the plasma membrane.

3.  The vitelline membrane hardens.

These changes ensure that fertilization of the egg by additional sperm is prevented.  They are part of the slow block to polyspermy.  The slow block is initiated within 10 seconds of the fusion of the plasma membranes of the two gametes.  For a visual of these steps, please follow this link for a Video of Slow Block to polyspermy.  The fusion of egg and sperm can be noted at the 2 o’clock position.

Back to Main Sea Urchin Page

References:

Marcey, David. “Chapter 13B. Animal Fertilization and Cleavage.” Lecture. Fertilization in Animal Development. Kenyon College Biology Department, Sept. 2006. Web. 1 Apr. 2011. <http://biology.kenyon.edu/courses/biol114/Chap13/Chapter_13B.html>.

Wong, Julian L., and Gary M. Wessel. “Major Components of a Sea Urchin Block to Polyspermy Are Structurally and Functionally Conserved.” Evolution and Development 6.3 (2004): 134-53. Print.

5 Responses to Fertilization: Avoiding Polyspermy

  1. Daniel J says:

    Does anyone know who the author of this article is? I would like to list it as one of my sources for my extended essay. Thank you in advance.

  2. gerald king says:

    Question:
    What is the percentage of human polyspermy successfully occurring, and what are the consequences of such union?

    • Jung Choi says:

      I don’t know of any documented cases of people born after polyspermy – having chromosomes from two sperm cells plus the egg would result in triploidy (having 3 copies of each chromosome rather than the normal 2 copies of each), which is an embryonic lethal condition, resulting in miscarriage or stillbirth.

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