The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo

parkin18

Fig. 4. KIN-18 acts via RHO-1 and is negatively regulated by PAR-3. (A) Stills from DIC time-lapse recordings of the genotypes indicated, PNM time is set to 0 s. White arrowheads indicate the site of pseudocleavage ingression. Control RNAi is pde-5(RNAi) (see Materials and methods for details). (B) Quantification of pseudocleavage persistence in the indicated genotypes. *p<0.01 with respect to kin-18(RNAi). °p<0.01 with respect to control RNAi; par-3(it71), note that the difference in pseudocleavage persistence between kin-18(RNAi) and par-3(it71); kin-18(RNAi) is not significant (p=0.51), and both are significantly different from wild type (p25, control RNAi;kin-18(RNAi) n=20, control RNAi;rho-1(RNAi) n=17, rho-1(RNAi);kin-18(RNAi) n=16). Averages are shown, error bars represent s.e.m., scale bars 10 μm.

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