The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo

untitled

Fig. 6. KIN-18 negatively regulates cortical localization of RHO-1. (A) Fluorescence pictures of embryos from control RNAi and kin-18(RNAi) in a GFP::RHO-1 expressing strain. (B) Quantification of relative cortical intensities in one cell embryos (from PNM to the appearance of the cytokinesis furrow) of the indicated genotypes. RHO-1 is enriched in the anterior in control embryos (*p<0.01 between anterior and posterior corteces). In KIN-18 depleted embryos, the posterior cortical levels of RHO-1 are significantly increased compared to the posterior in control embryos (°p<0.01). Anterior and posterior levels of GFP::RHO-1 in kin-18(RNAi) are not significantly different (p=0.38, control RNAi n=24, kin-18(RNAi) n=18). (C) Fluorescence pictures of embryos from control RNAi and rga-3/4(RNAi) of a GFP::RHO-1 expressing strain. (D) Quantification of relative cortical intensities in one cell embryos (from PNM to the appearance of the cytokinesis furrow) of the indicated genotypes. Anterior levels are significantly different from posterior levels in both control RNAi (**p<0.01) and rga-3/4(RNAi) embryos (p<0.02). The increase in levels observed at the anterior in rga-3/4(RNAi) compared to the anterior in control RNAi embryos is not significant (°°p=0.18) (control RNAi n=35, rga-3/4(RNAi) n=23). Averages are shown, error bars represent s.e.m., scale bars 10 μm

Bookmark the permalink.

Leave a Reply

Your email address will not be published. Required fields are marked *