RNA localization in the honeybee (Apis mellifera) ooctye reveals insights about the evolution of RNA localization mechanism

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Fig. 4. Localization of fluorescently labeled capped-RNAs in honeybee oocytes. In vitro synthesized transcribed RNAs labeled with UTP-488 were injected into the nurse cells of stage 2–4 oocytes and examined following incubated for 5 h at 35 1C. Expression of the endogenous transcript is shown in (C), (F), (I), and (L) panels for comparison. Amtsu488 in stage 5 oocytes becomes enriched to the anterior (A) and is clearly distributed at the anterior–dorsal pole by stage 8 ((B); arrow). Am-GB13806488 found through most of the stage 5 oocyte (D) but it was present mainly at the anterior–dorsal end of the oocyte by stage 8 ((E); arrow). Am-GB17282488 was also detected on one side of the oocyte ((G); arrow), becoming restricted to the posterior pole by stage 8 ((H); arrow). Labeled Am-PkaC1488 became quickly enriched to the posterior pole ((J) and (K); arrows). Honeybee Alexa-488 labeled transcripts were microinjected into the nurse cells of freshly dissected Drosophila egg chambers. Am-tsu488 was enriched to the anterior–dorsal region of the Drosophila oocyte ((M), arrow), as was Am-GB13806488 ((N), arrow). Am-PkaC1488 became enriched to the posterior ((O), arrow), as did Am-GB17282488 ((P), arrow). Honeybee oocytes are shown anterior to the left and dorsal side up. Stage 9 Drosophila egg chambers are shown anterior to the left and dorsal side up. Abbreviations: oocyte nucleus (ON).

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